Lect. Dr.Zaid Khudhur Mahmood

الدكتور زيد مدرس التحق بكلية النور الجامعة, قسم تقنيات التخدير في كانون الأول 2020 لتدريس مادة الأحياء الجزيئي النظري و مادة الوراثة البشرية النظري في قسم تقنيات المختبرات الطبية و مادة طرائق بحث النظري في قسم تقنيات صناعة الأسنان و مستمر لحد الأن بتدريس المدة الاولى و الثانية في قسم تقنيات المختبرات الطبية. بالإضافة الى الإشراف على بحوث التخرج للمرحلة المنتهية في قسم تقنيات المختبرات الطبية. حصل على شهادة الدكتوراة في علم تناسل الحيوان و وراثة الخلية من جامعة بوترا الماليزية تحت إشراف البروفيسور د. عبدالواحد هارون. حصل على درجة البكالوريوس و الماجستير من كلية الطب البيطري جامعة الموصل عامي 2004 و 2009 على التوالي. عمل كباحث مع أساتذة من كلية الطب البيطري و كلية العلوم جامعة الموصل بالإضافة الى عمله مع باحثيين من خارج العراق, من ماليزيا و اليمن و هولندا و كندا. شغل عدة مناصب كعضو في لجان متعددة منها اللجنة التنسيقية لقسم التخدير و لجنة اخلاقيات البحث العلمي في كلية النور الجامعة بالإصافة الى مشاركته في لجان الزيارات العلمية لتدريب الطلبة. شارك في برنامج دورات التعليم المستمر في كلية النور الجامعة. د. زيد مهتم بإجراء البحوث في مجال الفسلجة و تكاثر الحيوانات بشكل عام.

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Abstract :

Caseous lymphadenitis has been globally a prevalent disease among farmed small ruminants for over a century. As its chronic disease, sex hormone profiles and cellular changes of reproductive organs in infections associated with Corynebacterium pseudotuberculosisis and its exotoxin phospholipase D have been very few or absent. This study was conducted to acquire a better way of understanding the reproductive pathophysiology response of Corynebacterium pseudotuberculosisis and its exotoxin in mouse model. In this study, 64 healthy mice, 2-3 weeks of old, were divided equally into 3 groups, where the first group of mice were interperitoneally inoculated with 1.0 ml of sterile phosphate buffer solution (PBS), pH 7, the second group of mice were interperitoneally inoculated with 1.0 ml of 109 colony forming unit (CFU) of live C. pseudotuberculosis and the third group of mice were interperitoneally inoculated with 1.0 ml of single dose of exotoxin (PLD) extracted from C. pseudotuberculosis. Following infection, clinical signs were observed and blood samples were collected by cardiac puncture for sex hormone analysis. For microscopic examinations, the mice were euthanized using cervical dislocation approach and the reproductive organs were collected. The results revealed that there was no significant differences (p< 0.05) in the concentration of estrogen and progesterone levels between whole cell and exotoxin groups. Concentration of progesterone in PLD treated group (15.37±2.32 pg/ml) was lower than control group (17.61±2.91 pg/ml). The concentration of testosterone in C. pseudotuberculosis (2.98±3.70 pg/ml) was significantly (PLD).

Abstract :

Goat caseous lymphadenitis (CLA) is a chronic disease caused by Corynebacterium pseudotuberculosis. However, there is paucity of data about goat’s acute phase response during the course of CLA. This study was conducted to investigate the response of acute phase proteins, mainly haptoglobin (Hp), serum amyloid A (SAA) and the negative acute phase response, especially albumin after an experimental challenge of C. pseudotuberculosis and phospholipase D (PLD) in Cross bred Boer goats. Serum Hp concentration in goats challenged with C. pseudotuberculosis (inoculated with 1x109 cfu subcutaneously) showed a significant increase, 5 fold in males (0.98 ± 0.12 mg/ml) and 3 fold in females (0.66 ± 0.12 mg/ml) compared to the control (0.2 ± 0.02 mg/ml). Challenge with PLD (1 ml/20 kg body weight intravenously) also showed significant increase, 4 fold in males and females (0.89 ± 0.11 mg/ml; 0.82 ± 0.12 mg/ml) respectively compared to the control (0.2 ± 0.02 mg/ml). Albumin concentration showed a significant decrease in both treated groups compared to the control. There were no significant changes in SAA concentration between challenged and control goats. There was a significant response by Hp to C. pseudotuberculosis infection and PLD challenge. This was supported by the early acute response in which Hp was detected before CLA lesions were developed. Therefore, it concluded that C. pseudotuberculosis and PLD can influence the level of acute phase proteins in goats.

Abstract :

This study aims to assess the effect of Eurycoma longifolia aqueous extract on chilled and cryopreserved quality of bull sperm. Semen samples were obtained from four Simmental–Brangus. Each sample was divided into two fractions: the first fraction was used for chilling the semen, and the second fraction was used for the freezing process. Both fractions were extended with Tris–egg yolk extender supplemented with 0.0, 0.25, 0.5, 1.0, 2.5, 5.0, and 7.5 mg/ml Eurycoma longifolia aqueous extract. The diluted chilled fraction was chilled at 5 °C for 6 days, whereas the frozen–thawed fraction was frozen in liquid nitrogen. Data revealed that 1 mg/ml E. longifolia aqueous extract yielded significantly (p < .05) higher sperm motility, morphology, viability, and sperm membrane integrity compared with the control group and other treated groups in chilled semen evaluation. For cryopreserved sperm, a significant difference (p < .05) in sperm motility, viability, sperm membrane integrity, DNA integrity, and lipid peroxidation was observed between 5 mg/ml E. longifolia aqueous extract and other treated and control groups. However, no significant difference in the percentage of sperm exhibiting normal sperm morphology was observed among the groups. In conclusion, the addition of 0.25 and 1 mg/ml E. langifolia extract to chilled semen and 5 mg/ml E. longifolia aqueous extract to cryopreserved sperm into Tris–egg yolk extender helps in maintaining superior quality of bull spermatozoa during chilling and freezing.

Abstract :

The aim of this study was to investigate the effects of Corynebacterium pseudotuberculosis and phospholipase D (PLD) on buck's fertility, specifically, testosterone concentration, scrotal circumference and semen quality. Crossbred Boer goats (n=13) aged 12–14 months were divided into three groups. The first group (n=3) was inoculated with one ml sterile phosphate buffer saline subcutaneously into subaxillary area as the control. The second group (n=5) was inoculated with live C. pseudotuberculosis 1×109 cfu subcutaneously into subaxillary area. The third group (n=5) was inoculated with PLD 1 ml/20 kg BW intravenously into jugular vein. Blood collection was done twice a week over a period of three months for testosterone analyses. Semen was collected using electro-ejaculator once every two weeks whilst scrotal circumference was measured once every three weeks. Semen volume, semen pH and sperm progressive motility, concentration, live/dead percentage and morphology were evaluated. Bucks were euthanized three months' post inoculation and histopathological examination of the testicles and epididymis was performed. The results showed seven folds' significant decrease (P<0.05) in testosterone concentration in both C. pseudotuberculosis and PLD inoculated groups compared to the control. Semen volume, percentage of dead/live and abnormal sperm morphology showed significant increase (P<0.05) in both inoculated groups compared to the control. The following sperm defects were observed; tapered sperm, decapitated and knobbed head, thickened midpiece, distal and proximal droplet, sterilizing tail and folded tail. Scrotal circumference, semen pH, semen wave pattern, sperm motility and concentration showed significant decrease (P<0.05) in both inoculated groups compared to the control. The testicles showed varied degrees of degeneration and necrosis with shrunken seminferous tubules. In conclusion, testosterone concentration, scrotal circumference and semen quality were negatively affected in both inoculated groups. Moreover, percentages of live/dead and abnormal sperm morphology were also increased. The results suggest that both C. pseudotuberculosis and PLD had detrimental effects on buck's fertility.