Lect. Dr.Zaid Khudhur Mahmood

Dr. Zaid, a lecturer, joined Al-Noor University College, Department of Anesthesia Technologies, in December 2020 to teach Theoretical Molecular Biology and Theoretical Human Genetics in the Department of Medical Laboratory Technologies, as well as Theoretical Research Methods in the Department of Dental Prosthetics Techniques. He continues to teach the first and second terms in the Department of Medical Laboratory Technologies. Additionally, he supervises graduation research for the final stage in the Department of Medical Laboratory Technologies. Obtained his Ph.D. in Animal Reproduction and Cell Genetics from Universiti Putra Malaysia under the supervision of Professor Dr. Abdulwahid Haroun. He earned his Bachelor's and Master's degrees from the College of Veterinary Medicine, Mosul University, in 2004 and 2009 respectively. He worked as a researcher with professors from the College of Veterinary Medicine and the College of Science, Mosul University, as well as researchers from outside Iraq, including Malaysia, Yemen, the Netherlands, and Canada. Held several positions, including membership in various committees such as the Coordination Committee for the Anesthesia Department and the Research Ethics Committee at Al-Noor University College, in addition to his participation in scientific visitation committees for student training. He participated in the Continuous Education Courses program at Al-Noor University College. Dr. Zaid is interested in conducting research in the field of physiology and animal reproduction in general.

E.mail Address

[email protected]

Department

Department of Anesthesia Techniques

Specialization

PhD in Animal Reproduction and Cell Genetics

Number of published researches

22

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Most cited scientific research

Abstract :

Caseous lymphadenitis has been globally a prevalent disease among farmed small ruminants for over a century. As its chronic disease, sex hormone profiles and cellular changes of reproductive organs in infections associated with Corynebacterium pseudotuberculosisis and its exotoxin phospholipase D have been very few or absent. This study was conducted to acquire a better way of understanding the reproductive pathophysiology response of Corynebacterium pseudotuberculosisis and its exotoxin in mouse model. In this study, 64 healthy mice, 2-3 weeks of old, were divided equally into 3 groups, where the first group of mice were interperitoneally inoculated with 1.0 ml of sterile phosphate buffer solution (PBS), pH 7, the second group of mice were interperitoneally inoculated with 1.0 ml of 109 colony forming unit (CFU) of live C. pseudotuberculosis and the third group of mice were interperitoneally inoculated with 1.0 ml of single dose of exotoxin (PLD) extracted from C. pseudotuberculosis. Following infection, clinical signs were observed and blood samples were collected by cardiac puncture for sex hormone analysis. For microscopic examinations, the mice were euthanized using cervical dislocation approach and the reproductive organs were collected. The results revealed that there was no significant differences (p< 0.05) in the concentration of estrogen and progesterone levels between whole cell and exotoxin groups. Concentration of progesterone in PLD treated group (15.37±2.32 pg/ml) was lower than control group (17.61±2.91 pg/ml). The concentration of testosterone in C. pseudotuberculosis (2.98±3.70 pg/ml) was significantly (PLD).

Abstract :

Goat caseous lymphadenitis (CLA) is a chronic disease caused by Corynebacterium pseudotuberculosis. However, there is paucity of data about goat’s acute phase response during the course of CLA. This study was conducted to investigate the response of acute phase proteins, mainly haptoglobin (Hp), serum amyloid A (SAA) and the negative acute phase response, especially albumin after an experimental challenge of C. pseudotuberculosis and phospholipase D (PLD) in Cross bred Boer goats. Serum Hp concentration in goats challenged with C. pseudotuberculosis (inoculated with 1x109 cfu subcutaneously) showed a significant increase, 5 fold in males (0.98 ± 0.12 mg/ml) and 3 fold in females (0.66 ± 0.12 mg/ml) compared to the control (0.2 ± 0.02 mg/ml). Challenge with PLD (1 ml/20 kg body weight intravenously) also showed significant increase, 4 fold in males and females (0.89 ± 0.11 mg/ml; 0.82 ± 0.12 mg/ml) respectively compared to the control (0.2 ± 0.02 mg/ml). Albumin concentration showed a significant decrease in both treated groups compared to the control. There were no significant changes in SAA concentration between challenged and control goats. There was a significant response by Hp to C. pseudotuberculosis infection and PLD challenge. This was supported by the early acute response in which Hp was detected before CLA lesions were developed. Therefore, it concluded that C. pseudotuberculosis and PLD can influence the level of acute phase proteins in goats.

Abstract :

This study aims to assess the effect of Eurycoma longifolia aqueous extract on chilled and cryopreserved quality of bull sperm. Semen samples were obtained from four Simmental–Brangus. Each sample was divided into two fractions: the first fraction was used for chilling the semen, and the second fraction was used for the freezing process. Both fractions were extended with Tris–egg yolk extender supplemented with 0.0, 0.25, 0.5, 1.0, 2.5, 5.0, and 7.5 mg/ml Eurycoma longifolia aqueous extract. The diluted chilled fraction was chilled at 5 °C for 6 days, whereas the frozen–thawed fraction was frozen in liquid nitrogen. Data revealed that 1 mg/ml E. longifolia aqueous extract yielded significantly (p < .05) higher sperm motility, morphology, viability, and sperm membrane integrity compared with the control group and other treated groups in chilled semen evaluation. For cryopreserved sperm, a significant difference (p < .05) in sperm motility, viability, sperm membrane integrity, DNA integrity, and lipid peroxidation was observed between 5 mg/ml E. longifolia aqueous extract and other treated and control groups. However, no significant difference in the percentage of sperm exhibiting normal sperm morphology was observed among the groups. In conclusion, the addition of 0.25 and 1 mg/ml E. langifolia extract to chilled semen and 5 mg/ml E. longifolia aqueous extract to cryopreserved sperm into Tris–egg yolk extender helps in maintaining superior quality of bull spermatozoa during chilling and freezing.

Abstract :

The aim of this study was to investigate the effects of Corynebacterium pseudotuberculosis and phospholipase D (PLD) on buck's fertility, specifically, testosterone concentration, scrotal circumference and semen quality. Crossbred Boer goats (n=13) aged 12–14 months were divided into three groups. The first group (n=3) was inoculated with one ml sterile phosphate buffer saline subcutaneously into subaxillary area as the control. The second group (n=5) was inoculated with live C. pseudotuberculosis 1×109 cfu subcutaneously into subaxillary area. The third group (n=5) was inoculated with PLD 1 ml/20 kg BW intravenously into jugular vein. Blood collection was done twice a week over a period of three months for testosterone analyses. Semen was collected using electro-ejaculator once every two weeks whilst scrotal circumference was measured once every three weeks. Semen volume, semen pH and sperm progressive motility, concentration, live/dead percentage and morphology were evaluated. Bucks were euthanized three months' post inoculation and histopathological examination of the testicles and epididymis was performed. The results showed seven folds' significant decrease (P<0.05) in testosterone concentration in both C. pseudotuberculosis and PLD inoculated groups compared to the control. Semen volume, percentage of dead/live and abnormal sperm morphology showed significant increase (P<0.05) in both inoculated groups compared to the control. The following sperm defects were observed; tapered sperm, decapitated and knobbed head, thickened midpiece, distal and proximal droplet, sterilizing tail and folded tail. Scrotal circumference, semen pH, semen wave pattern, sperm motility and concentration showed significant decrease (P<0.05) in both inoculated groups compared to the control. The testicles showed varied degrees of degeneration and necrosis with shrunken seminferous tubules. In conclusion, testosterone concentration, scrotal circumference and semen quality were negatively affected in both inoculated groups. Moreover, percentages of live/dead and abnormal sperm morphology were also increased. The results suggest that both C. pseudotuberculosis and PLD had detrimental effects on buck's fertility.